transcription factor 3 atf3 monoclonal mouse anti atf3  (Santa Cruz Biotechnology)

Journal: Investigative ophthalmology & visual science

Article Title: De-Differentiation of Corneal Epithelial Cells Into Functional Limbal Epithelial Stem Cells After the Ablation of Innate Stem Cells.

doi: 10.1167/iovs.65.13.32

Figure Lengend Snippet: FIGURE 2. The regeneration of LESCs at the limbus after innate stem cells ablation. (A) Immunostaining of potential LESCs markers (CK14, deltaN-p63, p75NTR, CD63, TSPAN7, IFITM3, ATF3, and ApoE), Cx43 and CECs marker CK12 in frozen sections of normal cornea. (B-D) Immunostaining of Cx43, ApoE, and CK12 in frozen sections of normal cornea and LESCs-ablation cornea at indicated days after the limbal epithelial removal. The limbus was shown. (E) The percentage of ApoE+ cells at the limbus, and MFI (limbal basal cells)/MFI (peripheral basal cells) of Cx43 and CK12 were determined to indicate the recovery of LESCs. N, normal cornea. Scale bars = 50 μm. Note that the limbus is localized based on the location of iris and ciliary body in the bright field and the expressions of LESC markers.

Article Snippet: The following primary antibodies were used: rabbit anti-CK12 monoclonal antibody (Abcam, ab185627; 1:400), rabbit anti-CK7 monoclonal antibody (Abcam, ab181598; 1:400), rabbit anti-connexin 43 (Cx43) monoclonal antibody (CST, #3512; 1:80), rabbit anti-ApoE monoclonal antibody (Abcam, ab183596; 1:400), rabbit anti-CK14 monoclonal antibody (Abcam, ab119695; 1:200), rabbit anti-deltaN-p63 polyclonal antibody (BioLegend, 619002; 1:400), rabbit anti-p75NTR monoclonal antibody (CST, #8238; 1:800), rabbit anti-CD63 polyclonal antibody (Bioworld, BS72936; 1:400), rabbit anti-TSPAN7 polyclonal antibody (Proteintech, 18695-1-AP; 1:100), rabbit anti-IFITM3 monoclonal antibody (CST, #59212; 1:100), mouse anti-ATF3 monoclonal antibody (Santa Cruz, sc-518032; 1:100), rabbit anti-YAP monoclonal antibody (CST, #14074; 1:100), rabbit anti-active-YAP (non-phosphorylated YAP) monoclonal antibody (Abcam, ab205270; 1:200), rabbit anti-Ki67 monoclonal antibody (CST, #9129; 1:100), rabbit anti-p-Histone H3-Ser10 (pH3) monoclonal antibody (CST, #53348; 1:100), and mouse antiCK15 monoclonal antibody (Santa Cruz, sc-47697; 1:200).

Techniques: Immunostaining, Marker

Journal: International Journal of Molecular Sciences

Article Title: Gold and Cobalt Oxide Nanoparticles Modified Poly-Propylene Poly-Ethylene Glycol Membranes in Poly (ε-Caprolactone) Conduits Enhance Nerve Regeneration in the Sciatic Nerve of Healthy Rats

doi: 10.3390/ijms22137146

Figure Lengend Snippet: Boxplots of HSP27 and ATF3 immunoreactivity of dorsal root ganglia (DRG) after nerve reconstruction of a 10 mm long nerve defect in rat sciatic nerve is presented. The groups are coded according to the membrane properties, i.e., Hollow: hollow conduit, PPEG: PCL conduit with PPEG membrane, AuPPEG: PCL conduit with AuPPEG membrane, AuCoOPPEG: PCL conduit with AuCoOPPEG membrane. Box plots indicate the 25 and 75 percentiles (Tukey’s Hinge) with the horizontal line in the middle indicating the median value. Error bars show min-max values. ( A ) HSP27 immunoreactivity data in experimental and unoperated (control) side DRGs, ( B ) ATF3 immunoreactivity data in experimental DRGs. Cont: DRGs on the unoperated-control side, Exp: DRGs on the experimental side.

Article Snippet: The following primary antibodies were used: monoclonal mouse anti-human neurofilament (1:80; Dako, Glostrup, Denmark), for axonal outgrowth; polyclonal goat anti-HSP-27(1:200, Santa Cruz Biotechnology) for detection of HSP27 in Schwann cells and axons in sciatic nerve; polyclonal rabbit anti-HSP-27 (1:200, Enzo, Farmingdale, NY, USA) for sensory neurons and axons in DRG; monoclonal mouse anti-ATF3 for activated Schwann cells and for sensory neurons in DRG (1:200, Santa Cruz Biotechnology); and monoclonal rabbit anti-cleaved caspase 3 for apoptotic Schwann cells (1:200, Cell signalling Technology) [ ].

Techniques: Membrane, Control

Journal: International Journal of Molecular Sciences

Article Title: Gold and Cobalt Oxide Nanoparticles Modified Poly-Propylene Poly-Ethylene Glycol Membranes in Poly (ε-Caprolactone) Conduits Enhance Nerve Regeneration in the Sciatic Nerve of Healthy Rats

doi: 10.3390/ijms22137146

Figure Lengend Snippet: ATF3 immunoreactivity in whole DRG in hollow PCL conduits, ( A ) control, ( B ) experimental; PCL conduit with PPEG membrane, ( C ) control, ( D ) experimental; PCL conduit with AuPPEG membrane, ( E ) control, ( F ) experimental; and in PCL conduit with AuCoOPPEG membrane, ( G ) control, ( H ) experimental. Bar = 100 µm.

Article Snippet: The following primary antibodies were used: monoclonal mouse anti-human neurofilament (1:80; Dako, Glostrup, Denmark), for axonal outgrowth; polyclonal goat anti-HSP-27(1:200, Santa Cruz Biotechnology) for detection of HSP27 in Schwann cells and axons in sciatic nerve; polyclonal rabbit anti-HSP-27 (1:200, Enzo, Farmingdale, NY, USA) for sensory neurons and axons in DRG; monoclonal mouse anti-ATF3 for activated Schwann cells and for sensory neurons in DRG (1:200, Santa Cruz Biotechnology); and monoclonal rabbit anti-cleaved caspase 3 for apoptotic Schwann cells (1:200, Cell signalling Technology) [ ].

Techniques: Control, Membrane

Journal: International Journal of Molecular Sciences

Article Title: Gold and Cobalt Oxide Nanoparticles Modified Poly-Propylene Poly-Ethylene Glycol Membranes in Poly (ε-Caprolactone) Conduits Enhance Nerve Regeneration in the Sciatic Nerve of Healthy Rats

doi: 10.3390/ijms22137146

Figure Lengend Snippet: Scatter plots for axonal outgrowth (neurofilament staining in µm) against the percentage of HSP27 immunoreactivity, percentage of ATF3 and cleaved caspase 3 in the regenerated matrix and the percentage of ATF3 immunoreactivity in DRG after reconstruction of a 10 mm nerve defect in rat sciatic nerve is presented. For r- and p -values see the results section.

Article Snippet: The following primary antibodies were used: monoclonal mouse anti-human neurofilament (1:80; Dako, Glostrup, Denmark), for axonal outgrowth; polyclonal goat anti-HSP-27(1:200, Santa Cruz Biotechnology) for detection of HSP27 in Schwann cells and axons in sciatic nerve; polyclonal rabbit anti-HSP-27 (1:200, Enzo, Farmingdale, NY, USA) for sensory neurons and axons in DRG; monoclonal mouse anti-ATF3 for activated Schwann cells and for sensory neurons in DRG (1:200, Santa Cruz Biotechnology); and monoclonal rabbit anti-cleaved caspase 3 for apoptotic Schwann cells (1:200, Cell signalling Technology) [ ].

Techniques: Staining

Journal: International Journal of Molecular Sciences

Article Title: Gold and Cobalt Oxide Nanoparticles Modified Poly-Propylene Poly-Ethylene Glycol Membranes in Poly (ε-Caprolactone) Conduits Enhance Nerve Regeneration in the Sciatic Nerve of Healthy Rats

doi: 10.3390/ijms22137146

Figure Lengend Snippet: The used primary and secondary antibodies for immunohistochemical analysis are listed for sciatic nerve and DRG.

Article Snippet: The following primary antibodies were used: monoclonal mouse anti-human neurofilament (1:80; Dako, Glostrup, Denmark), for axonal outgrowth; polyclonal goat anti-HSP-27(1:200, Santa Cruz Biotechnology) for detection of HSP27 in Schwann cells and axons in sciatic nerve; polyclonal rabbit anti-HSP-27 (1:200, Enzo, Farmingdale, NY, USA) for sensory neurons and axons in DRG; monoclonal mouse anti-ATF3 for activated Schwann cells and for sensory neurons in DRG (1:200, Santa Cruz Biotechnology); and monoclonal rabbit anti-cleaved caspase 3 for apoptotic Schwann cells (1:200, Cell signalling Technology) [ ].

Techniques: Immunohistochemical staining

Journal: International Journal of Molecular Sciences

Article Title: Gold and Cobalt Oxide Nanoparticles Modified Poly-Propylene Poly-Ethylene Glycol Membranes in Poly (ε-Caprolactone) Conduits Enhance Nerve Regeneration in the Sciatic Nerve of Healthy Rats

doi: 10.3390/ijms22137146

Figure Lengend Snippet: The used primary and secondary antibodies for immunohistochemical analysis are listed for sciatic nerve and DRG.

Article Snippet: , Activating transcription factor 3 (ATF3) , Monoclonal mouse anti-ATF3 (1:200, Santa Cruz Biotechnology, Dallas, TX, USA) , Alexa Fluor 488-goat anti-mouse IgG (1:500, Invitrogen, Molecular Probes, Eugene, OR, USA).

Techniques: Immunohistochemical staining

Journal: Scientific Reports

Article Title: Alteration of the lysophosphatidic acid and its precursor lysophosphatidylcholine levels in spinal cord stenosis: A study using a rat cauda equina compression model

doi: 10.1038/s41598-019-52999-5

Figure Lengend Snippet: Establishment of a rat model of LSS or CEC. ( a ) A schematic drawing of the rat spinal cord, the location of the inserted silicon blocks (the L3 and L5 epidural space) and the collection of tissue for further analysis. ( b ) Rotarod was used to test motor function before surgery and on days 1, 7, 14 and 28 after surgery. Compared to that in the sham-operated rats, the duration of running time was significantly (* p < 0.05) decreased in the CEC model rats beginning on the first day after surgery. ( c ) A representative image of IHC staining of the L5 DRG after the placement of the 2 silicon blocks with an ATF3 antibody showing the strong expression of ATF3 (arrowheads) in the nuclei (400×). Upper 2 images (40×) show the origin of the zoom-in DRGs. Dapi staining (lower) for co-localization of the ATF3 stained nucleus. The ATF3-positive cells on both sides of the L5 DRG were counted, and the calculated percentage of positive cells is shown (n = 5). ATF3 was most increased in the CEC rats on day 1 after surgery. On day 7 and day 14, only a few nuclei were labeled with ATF3 in the CEC rats. ATF3 mRNA expression levels in the DRGs at the epicenter (L3, L4 and L5) were also compared for each time point (n = 5). The percentage of ATF3-positive cells and the ATF3 mRNA expression were significantly (*p < 0.05) increased in the CEC rats on day 1. ( d ) Flow cytometry analysis showed the infiltration of CD11b/CD45 double-positive cells into the spinal cord after surgery. As a control, we used tissues from the same regions from naive and sham-operated rats. The results from the rostral segments (above the silicon blocks) and the epicenter segments (where the 2 silicon blocks were inserted) of the spinal cord tissue from the CEC rats on days 1, 7, 14 and 28 are shown. The percentage of CD11b/CD45 double-positive cells (upper panel) in the epicenter segments was markedly (*p < 0.05) enhanced, indicating the infiltration of macrophages and microglia into the site of the injury. Representative forward/scatter dot plots showing the percentage of CD11b/CD45 double-positive cells (lower panel).

Article Snippet: The sections were immunostained with a mouse monoclonal antibody against ATF3 (1:300, Santa Cruz Biotechnology, Texas, USA) as the primary antibody and with a goat anti-mouse Alexa Fluor 594 (1:500, Thermo Fisher Scientific, Massachusetts, USA) antibody as the secondary antibody, and Vectashield mounting medium (Vector Laboratories, CA, USA) was used for coverslipping.

Techniques: Immunohistochemistry, Expressing, Staining, Labeling, Flow Cytometry, Control

Journal: Frontiers in Cellular Neuroscience

Article Title: The ATP-P2X7 Signaling Pathway Participates in the Regulation of Slit1 Expression in Satellite Glial Cells

doi: 10.3389/fncel.2019.00420

Figure Lengend Snippet: Primary and secondary antibodies used in this study.

Article Snippet: , Mouse monoclonal anti-ATF3 , Abcam , Ab58668 , 1:100 IF.

Techniques: Control